The bacteria that live inside our digestive tract undoubtedly play a vital part in our health. But buyer beware of companies that claim to have deciphered the gut microbiome. Research out today shows that no two at-home tests will tell you the same thing.
Government scientists sent standardized fecal samples to seven different gut health testing companies. The companies returned results that varied from one another, sometimes dramatically, while one company’s tests couldn’t conclusively decide if the same samples belonged to a healthy microbiome or not. The findings indicate that customers shouldn’t put too much stock in these tests, at least right now, the researchers say.
“Our results demonstrate the need for standards to ensure analytical validity and consumer confidence,” the authors wrote in their paper, published Thursday in Communications Biology.
Not quite there yet
Exciting as the field of gut health is, it’s very much in its infancy. We’re still not quite sure exactly what makes for a healthy mix of bacteria in our guts, much less how to reliably fix an unhealthy microbiome (it’s likely there are many different combinations of bacteria that could be “healthy”). And we’re still trying to untangle the complex interactions between our gut bacteria and various health conditions.
This uncertainty hasn’t stopped several companies from entering the direct-to-consumer industry, however. While some may be cautious in their advertising, others have claimed their tests can tell whether a person’s microbiome is healthy, and they might even sell products that will supposedly restore a dysfunctional one. Many scientists have already called for tighter regulation of these tests. Researchers at the National Institute of Standards and Technology, a division of the U.S. Department of Commerce, and others sought to gauge the reliability of these tests across different companies.
Our poop can be used as a marker for the gut microbiome, but it has its flaws. There’s lots of inherent variability in the samples (aka, turds) you use for testing, such that two samples given a day apart can yield noticeably different compositions of bacteria. And even the bacteria found at the beginning of a sample can differ significantly from the bacteria found at the end of one. Companies can also have important differences in how they collect, analyze, and interpret the samples.
To get around some of these limitations, NIST scientists developed and released a human stool reference material last year. This material has a consistent mix of bacteria and metabolic byproducts, which have been extensively categorized by the NIST. It’s important to note that this stool isn’t meant to be the gold standard of a healthy microbiome, only a baseline that scientists can use for research and testing. Though the final version contains a blend of samples from eight healthy donors, the NIST also created mixtures from individual donors.
In this latest study, the researchers used a single donor’s standardized poop to vet the accuracy of testing kits from seven DTC gut health companies (none of the companies are named in the study). For each company, they provided three samples of the same poop.
The companies’ tests routinely disagreed with one another, they found. While one company stated that a sample was filled with Clostridium bacteria (a common group of gut bacteria, some of which can cause disease), for instance, three others failed to detect traces of it in one or more samples. One company also offered contradictory assessments of the same poop. It declared one sample to be “unhealthy,” yet the other two were found to be “healthy.” All in all, they determined that the variability between companies was about as wide as the variability you’re likely to find between different donors.
These varying test results are likely due to differences in methods for sample collection and analysis used by the companies. And it suggests that these tests can’t be relied on to tell you much of anything currently. That lack of reliability could be harmful, too.
While some people might take these tests out of simple curiosity, others will use them to guide their future health decisions. At a press briefing Wednesday, co-author Diane Hoffman, a professor of health care law at the University of Maryland, detailed having surveyed doctors on how their patients have used these tests. In some cases, people radically changed their diets (or the diets of their children) in possibly dangerous ways based on test results; in one case, a person even underwent a fecal microbiota transplant, a promising but still developing treatment for changing the gut microbiome.
Other consumers could end up getting swindled by less scrupulous companies.
“Some of these companies also offer probiotics and other dietary supplements that they say will correct your microbiome,” said co-author Scott Jackson, a now-retired NIST researcher, in a response to a question from Gizmodo at the press briefing. “To correct your microbiome would presume that they’re seeing something wrong with your microbiome. More times than not, it just so happens that the thing that’s wrong with your microbiome happens to complement the product that they also sell.”
Should you take these tests?
There have been genuinely important advances in understanding the gut microbiome lately. And down the line, doctors could possibly offer treatments and interventions that can consistently help improve the gut health of many people. But that potential will depend on scientists and companies developing consistent standards across the field, both for testing and research, the authors say.
For now, these DTC gut health tests simply aren’t ready for primetime. And much like consumer genetic tests, the average person is probably better off staying far away from any tests claiming to have figured out the gut.
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![‘The Ghost in the Shell’ Director, Character Designer on Keeping Motoko True to the Manga
Dan Da Dan anime studio Science SARU’s adaptation of The Ghost in the Shell is finally streaming on Prime Video, and its pilot episode looks like a ’90s anime fever dream in the best way possible. Major Motoko Kusanagi kicks all the ass, its soundtrack is a bop, and its action sequences are a chef’s kiss. Don’t just take our word for it; check out the trailer below and see for yourself how Science SARU cooked once again. [embed]https://www.youtube.com/watch?v=ahZn4dVCbvs[/embed] Ahead of its premiere, io9 got to chat with director Mokochan and character designer Shuhei Handa at Anime Expo about how their vision for Masamune Shirow’s seminal cyberpunk manga came to be, as well as a couple of pointed fandom-centric questions for fans who’ve been dying to see Major Motoko Kusanagi realized in all her goofy glory. Left: Shuhei Handa, right: Mokochan. © Isaiah Colbert/io9 This interview was edited for clarity. Isaiah Colbert, io9: From Keep Your Hands Off Eizouken!, Devilman Crybaby, Inu-Oh, Scott Pilgrim Takes Off, and Dan Da Dan to Sanda and Jaadugar: A Witch in Mongolia, Science SARU has built an impressive portfolio and reputation for making bold, expressive, and experimental anime. What about The Ghost in the Shell made it feel like a natural fit as the studio’s next project?
Mokochan: For all of the works done by Science SARU, using animation to do storytelling is very important. That’s why we use a lot of hand-drawn animation in it. For The Ghost in the Shell, the most important thing is to use the visuals to capture its world. It’s the same policy with other series as well.
TVアニメ「#攻殻機動隊 THE GHOST IN THE SHELL」⋱ご視聴ありがとうございました⋰ ◤◢◤◢◤◢◤◢◤◢◤◢◤◢ 第01話より草薙素子と荒巻大輔の出会い 👩🏻 🐵迫力の原画を大公開! 原画|小島崇史 さん ◤◢◤◢◤◢◤◢◤◢◤◢◤◢https://t.co/wICYD3OlUm pic.twitter.com/6rNlJdXZZk — サイエンスSARU (@sciencesaru) July 7, 2026 io9: From the jump, The Ghost in the Shell marks a stark tonal shift—a return—towards Masamune Shirow’s lighter, more mischievous vision of the cyberpunk epic. What led Science SARU to pivot away from the austere, military tone that Mamoru Oshii’s 1995 film would go on to cement as the franchise’s vibe for decades? Mokochan: We didn’t intentionally try to change the tone from how director Mamoru Oshii did previously because the concept for making this new series is to base it on the original manga, so we already had our own way of making this one.
© Masamune Shirow/Kodansha io9: In an era when people often use AI to cheaply mimic retro anime aesthetics, the new Ghost in the Shell feels like a deliberate rejection of AI’s pervasiveness in the arts—where every frame in the trailers looks unmistakably hand‑crafted. It’s a phenomenon that’s certainly paradoxical, considering that the manga prophesied how such technologies would be utilized in the not-so-far-off year of 2029. What’s Science SARU’s stance on AI, and what specific animation techniques did it use most to capture the show’s aesthetic through the power of collective human artistry? Mokochan: One of the concepts of the original manga is about the mysterious part of the human being. So we wanted to honor how the original creator captured the world and how we express all of the story in the original manga. So we wanted to follow the same concept using the same method to show humanity by using hand-drawn art. We prefer to just use our arms to draw all of the animation. Handa: It’s because the main concept is about the human body, so that’s why we prefer hand-drawn art over AI.
© Science SARU io9: Major Kusanagi has long been read as a bisexual icon in the West, especially because of Shirow’s famous island-escapade chapter with her gal pals, which has been lost in some Western reprints of the original manga. She’s also considerably goofier in the manga, a trait fans lovingly call a “bisexual disaster.” In what ways was it important for Science SARU to depict Kusanagi’s inherent goofball nature and her queerness in this adaptation, and what does portraying that side of her mean for a global audience in 2026? Mokochan: During the process of making the new series, we were actually very surprised by it because, compared to the original manga, a lot of the thoughts about sexuality are very advanced, exploring many possibilities. It’s not just about sexuality; it’s also about the human being, technology, and many other things. [The manga] accepts everything in the world. That was a very big surprise. We didn’t intend to modernize the content to make it fit the new era.
© Science SARU [The Ghost in the Shell producers’ note in regard to Science SARU’s adaptation of Motoko’s vacation scene from the original manga: “We had to adjust the depictions in the original work to match the rating.”] Handa: When we portray Kusanagi’s character, we like to bring out all the comical parts because they’re in the original manga. It’s kind of different how director Oshii portrays her in the previous series because, in those series, Kusanagi was not that expressive. We prefer to keep the authentic parts of the original manga, so we thought we definitely needed to keep the comical parts in the character, so that’s why we included that content. © Science SARU © Science SARU © Science SARU © Science SARU io9: As a fan of the series, I’ve seen Ghost in the Shell go through different iterations, reinterpretations, and reboots. And in that, Motoko’s design has changed in a way that reflects how different people interpret her. But while her outer shell is different, the themes—the ghost in the original story—remain in each adaptation. For Science SARU’s newer adaptation of The Ghost in the Shell, which themes from the original manga feel more prescient now than when it first came out?
Mokochan: Because the original intent of this project is to follow the original manga—not just Kusanagi, but also all the content and the world’s concept—we didn’t try to modernize any part of it. The creative team, all of whom are also big fans of the original manga. So, for the world, the story, the characters, and the theme, it’s all the same because we want to honor the original manga. ウオオオ攻殻機動隊1話にLOと作画監督で参加させていただきました!憧れの攻殻機動隊に関わることができて感激です🔥LOはセンターの子供が撃たれるあたりから脱走するところまで、作監は冒頭からOP前あたりまでです。少佐かっこよくて可愛かった〜!こちら担当LOのうちの1カットです🙏🏻#攻殻機動隊 pic.twitter.com/zUlxgD9fin — 宮かなえ (@miyakanae_) July 7, 2026 The Ghost in the Shell is streaming now on Prime Video. io9 is on the ground at Anime Expo 2026. We’ll be bringing you updates on all the biggest panels, screenings, and announcements, plus exclusive one-on-one interviews with the people behind some of the best and most popular anime around. You can check out all of io9’s Anime Expo coverage here. Want more io9 news? Check out when to expect the latest Marvel, Star Wars, and Star Trek releases, what’s next for the DC Universe on film and TV, and everything you need to know about the future of Doctor Who. #Ghost #Shell #Director #Character #Designer #Keeping #Motoko #True #MangaAnime,Anime Expo,Science Saru,The Ghost in the Shell ‘The Ghost in the Shell’ Director, Character Designer on Keeping Motoko True to the Manga
Dan Da Dan anime studio Science SARU’s adaptation of The Ghost in the Shell is finally streaming on Prime Video, and its pilot episode looks like a ’90s anime fever dream in the best way possible. Major Motoko Kusanagi kicks all the ass, its soundtrack is a bop, and its action sequences are a chef’s kiss. Don’t just take our word for it; check out the trailer below and see for yourself how Science SARU cooked once again. [embed]https://www.youtube.com/watch?v=ahZn4dVCbvs[/embed] Ahead of its premiere, io9 got to chat with director Mokochan and character designer Shuhei Handa at Anime Expo about how their vision for Masamune Shirow’s seminal cyberpunk manga came to be, as well as a couple of pointed fandom-centric questions for fans who’ve been dying to see Major Motoko Kusanagi realized in all her goofy glory. Left: Shuhei Handa, right: Mokochan. © Isaiah Colbert/io9 This interview was edited for clarity. Isaiah Colbert, io9: From Keep Your Hands Off Eizouken!, Devilman Crybaby, Inu-Oh, Scott Pilgrim Takes Off, and Dan Da Dan to Sanda and Jaadugar: A Witch in Mongolia, Science SARU has built an impressive portfolio and reputation for making bold, expressive, and experimental anime. What about The Ghost in the Shell made it feel like a natural fit as the studio’s next project?
Mokochan: For all of the works done by Science SARU, using animation to do storytelling is very important. That’s why we use a lot of hand-drawn animation in it. For The Ghost in the Shell, the most important thing is to use the visuals to capture its world. It’s the same policy with other series as well.
TVアニメ「#攻殻機動隊 THE GHOST IN THE SHELL」⋱ご視聴ありがとうございました⋰ ◤◢◤◢◤◢◤◢◤◢◤◢◤◢ 第01話より草薙素子と荒巻大輔の出会い 👩🏻 🐵迫力の原画を大公開! 原画|小島崇史 さん ◤◢◤◢◤◢◤◢◤◢◤◢◤◢https://t.co/wICYD3OlUm pic.twitter.com/6rNlJdXZZk — サイエンスSARU (@sciencesaru) July 7, 2026 io9: From the jump, The Ghost in the Shell marks a stark tonal shift—a return—towards Masamune Shirow’s lighter, more mischievous vision of the cyberpunk epic. What led Science SARU to pivot away from the austere, military tone that Mamoru Oshii’s 1995 film would go on to cement as the franchise’s vibe for decades? Mokochan: We didn’t intentionally try to change the tone from how director Mamoru Oshii did previously because the concept for making this new series is to base it on the original manga, so we already had our own way of making this one.
© Masamune Shirow/Kodansha io9: In an era when people often use AI to cheaply mimic retro anime aesthetics, the new Ghost in the Shell feels like a deliberate rejection of AI’s pervasiveness in the arts—where every frame in the trailers looks unmistakably hand‑crafted. It’s a phenomenon that’s certainly paradoxical, considering that the manga prophesied how such technologies would be utilized in the not-so-far-off year of 2029. What’s Science SARU’s stance on AI, and what specific animation techniques did it use most to capture the show’s aesthetic through the power of collective human artistry? Mokochan: One of the concepts of the original manga is about the mysterious part of the human being. So we wanted to honor how the original creator captured the world and how we express all of the story in the original manga. So we wanted to follow the same concept using the same method to show humanity by using hand-drawn art. We prefer to just use our arms to draw all of the animation. Handa: It’s because the main concept is about the human body, so that’s why we prefer hand-drawn art over AI.
© Science SARU io9: Major Kusanagi has long been read as a bisexual icon in the West, especially because of Shirow’s famous island-escapade chapter with her gal pals, which has been lost in some Western reprints of the original manga. She’s also considerably goofier in the manga, a trait fans lovingly call a “bisexual disaster.” In what ways was it important for Science SARU to depict Kusanagi’s inherent goofball nature and her queerness in this adaptation, and what does portraying that side of her mean for a global audience in 2026? Mokochan: During the process of making the new series, we were actually very surprised by it because, compared to the original manga, a lot of the thoughts about sexuality are very advanced, exploring many possibilities. It’s not just about sexuality; it’s also about the human being, technology, and many other things. [The manga] accepts everything in the world. That was a very big surprise. We didn’t intend to modernize the content to make it fit the new era.
© Science SARU [The Ghost in the Shell producers’ note in regard to Science SARU’s adaptation of Motoko’s vacation scene from the original manga: “We had to adjust the depictions in the original work to match the rating.”] Handa: When we portray Kusanagi’s character, we like to bring out all the comical parts because they’re in the original manga. It’s kind of different how director Oshii portrays her in the previous series because, in those series, Kusanagi was not that expressive. We prefer to keep the authentic parts of the original manga, so we thought we definitely needed to keep the comical parts in the character, so that’s why we included that content. © Science SARU © Science SARU © Science SARU © Science SARU io9: As a fan of the series, I’ve seen Ghost in the Shell go through different iterations, reinterpretations, and reboots. And in that, Motoko’s design has changed in a way that reflects how different people interpret her. But while her outer shell is different, the themes—the ghost in the original story—remain in each adaptation. For Science SARU’s newer adaptation of The Ghost in the Shell, which themes from the original manga feel more prescient now than when it first came out?
Mokochan: Because the original intent of this project is to follow the original manga—not just Kusanagi, but also all the content and the world’s concept—we didn’t try to modernize any part of it. The creative team, all of whom are also big fans of the original manga. So, for the world, the story, the characters, and the theme, it’s all the same because we want to honor the original manga. ウオオオ攻殻機動隊1話にLOと作画監督で参加させていただきました!憧れの攻殻機動隊に関わることができて感激です🔥LOはセンターの子供が撃たれるあたりから脱走するところまで、作監は冒頭からOP前あたりまでです。少佐かっこよくて可愛かった〜!こちら担当LOのうちの1カットです🙏🏻#攻殻機動隊 pic.twitter.com/zUlxgD9fin — 宮かなえ (@miyakanae_) July 7, 2026 The Ghost in the Shell is streaming now on Prime Video. io9 is on the ground at Anime Expo 2026. We’ll be bringing you updates on all the biggest panels, screenings, and announcements, plus exclusive one-on-one interviews with the people behind some of the best and most popular anime around. You can check out all of io9’s Anime Expo coverage here. Want more io9 news? Check out when to expect the latest Marvel, Star Wars, and Star Trek releases, what’s next for the DC Universe on film and TV, and everything you need to know about the future of Doctor Who. #Ghost #Shell #Director #Character #Designer #Keeping #Motoko #True #MangaAnime,Anime Expo,Science Saru,The Ghost in the Shell](https://gizmodo.com/app/uploads/2026/07/The-Ghost-in-the-Shell-Mokachan-Shuhei-Handa.jpg)







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